Single nuclei RNA sequencing (snRNAseq) was performed on frozen liver samples from male C57BL/6 mice gavaged with sesame oil control or 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) every 4 days for 28 days. Approximately 19,907 hepatic genes were detected across 16,015 sequenced nuclei from sesame oil control and 30 μg/kg TCDD treated samples. On day 28 (PND 56) livers were immediately collected, frozen in liquid nitrogen, and stored at -80°C. Nuclei were isolated from frozen livers, stained with DAPI, and subjected to FACS in order to generate a single nuclei suspension which was loaded onto the 10X Genomics Chromium controller for library generation using the Single Cell 3' V3 library preparation protocol.

Nault R, Fader KA, Bhattacharya S, Zacharewski TR. Single-Nuclei RNA Sequencing Assessment of the Hepatic Effects of 2,3,7,8-Tetrachlorodibenzo-p-dioxin. Cell Mol Gastroenterol Hepatol 2021;11(1):147-159. PMID: 32791302 DOI: 10.1016/j.jcmgh.2020.07.012

Raw data is deposited in the Gene Expression Omnibus: GSE148339