Microvascular integrity of human and murine tissues, including the kidney, intestine, and lungs, relies on the membrane-associated glycoprotein plasmalemma vesicle-associated protein (PLVAP). To resolve hepatic stellate cell-specific Plvap KO induced transcriptional changes  in individual liver cell populations, we conducted snRNA-seq of livers from fasted PlvapHSC-KO mice and littermate controls (n = 3). Nuclei clustered into 11 clusters representing major cell populations and each containing nuclei from all biological replicates across genotypes. PLVAP transcripts were found in HSC nuclei, and HSC PLVAP ablation led to no changes in cell-type abundances. Comparing hepatocyte nuclei by genotype, we identified DEGs implicated in lipid metabolism and transport.