ACE2 and TMPRSS2 most enriched within GSTA1+MGST3+ absorptive enterocytes in context of non-inflamed terminal ileumBetty Zheng*, Alison Yu*, Benjamin Doran*, Kayla Cribben, Ryan Fleming, Alexandria Narayan, Kayla Betz, Brandi Bratrude, Victor Tkachev, Alex Shalek#, Jose Ordovas-Montanes#, Leslie Kean#We collected single-cell RNA-seq data from biopsies of the human terminal ileum (epithelial and lamina propria) across 13 children diagnosed with functional gastrointestinal disease (without inflammation) and ranging in age 6-18 years old. Ileal absorptive epithelial cells appear significantly enriched for ACE2 and TMPRSS2 expression. Furthermore, we identify a subset (888 cells, ~6.5% of epithelial cells) which co-express both genes. By identifying rare co-expressing cells, we are... (continued)

Barrier tissue dysfunction is a fundamental feature of chronic human inflammatory diseases1. Specialized subsets of epithelial cells—including secretory and ciliated cells—differentiate from basal stem cells to collectively protect the upper airway. Allergic inflammation can develop from persistent activation of type 2 immunity in the upper airway, resulting in chronic rhinosinusitis, which ranges in severity from rhinitis to severe nasal polyps. Basal cell hyperplasia is a hallmark of severe disease, but it is not known how these progenitor cells contribute to clinical presentation and barrier tissue dysfunction in humans. Here we profile primary human surgical chronic rhinosinusitis samples (18,036 cells, n = 12)... (continued)

We used previously unpublished NHP (Macaca mulatta) lung and small intestinal ileal tissue collected following necropsy of healthy adult animals with profiled with Seq-Well v1 to assess which cell types tissues represent natural targets of SARS-CoV-2. We saw that epithelial cells express ACE2 or co-express ACE2 and TMPRSS2. Note that this is a pre-publication dataset, so in this study we include expression of ACE2 and TMPRSS2 across all cells and expression of all genes in the ACE2+ subsets: epithelial cells of the lung and enterocytes in the ileum. Contributors (listed alphabetically): Shaina L. Carroll, Lucrezia Colonna, Leslie S. Kean, Alex K. Shalek,... (continued)

Epithelial cells were computationally identified from scRNA-Seq from samples of tissue consisting of granuloma and adjacent, uninvolved lung samples from 10 Mtb-infected NHPs were collected with Seq-Well S^3. Here we show Type 1 Pneumocytes, Type 2 Pneumocytes, Secretory, and Multiciliated subsets of these single cells to facilitate inquiry into the susceptibility of these cells to respiratory viruses, specifically COVID-19. We find that Type 2 Pneumocytes are enriched for cells that co-express the COVID-19 receptor (ACE2) and associated protease (TMRSS2) and these cells are more frequently found in samples from granulomatous regions of the lung. Note that since this is a pre-publication... (continued)

This study contains the epithelial cells identified in a scRNA-seq dataset from lung tissue derived from surgical resection collected with Seq-Well S^3. We include for further investigation the epithelial subsets of the lung tissue which were the only subsets of cells in this dataset to express ACE2 and TMPRSS2. For all other cell subsets, we include only the expression of ACE2 and TMPRSS2. Note that since this is a pre-publication dataset, we only include cell types which express ACE2 and metadata relevant to this inquiry. The full dataset will be released at a later date. Contributors (listed alphabetically):  Alasdair Leslie, Ian Mbano, Alex K.... (continued)