Bronchiolitis Obliterans Syndrome Lung scRNA-Seq

In as many as half of all lung transplant patients, Bronchiolitis Obliterans Syndrome or BOS, can develop, resulting in a progress deterioration of lung function. In this experiment, scRNA-Seq was performed on a series of 5 distal and proximal airway regions of the removed lung, prior to transplantation. The patient was a a male 9 year old, with BOS confirmed upon biopsy, which occurred after a bone marrow transplantation (graft versus host rejection). The sample was prepared from flash frozen sample, processed using a cold activated protease protocol in the laboratory of Anne Karina  Perl (Cincinnati Children's Hospital - IRB 2013-3309). The dataset was processed using the Terra Optimus and Cumulus pipelines to produce unsupervised louvain clusters as well as in the software ICGS2 (unsupervised) and cellHarmony (supervised) to identify cell population identities relative to prior published studies. For additional information, contact: anne.perl@cchmc.org