Lungs and lymph nodes were harvested from C57Bl/6J mice (male, 12 weeks). The tissues were digested with collagenase IV, followed by passage through a 70um cell strainer and red blood cell lysis. For FACS, the cells were stained with monoclonal antibodies and sorted (CD45+, lin-, MHCII+, CD11c+). The sorted cells were taken for encapsulation and single-cell RNAseq using the 10x Chromium platform.

This data set was taken from Maier et al., Nature (2020).