Spleen, blood, IELs, kidney, salivary gland, fat and liver were harvested from CD8a-APCFlour780 IV injected C57BL/6J mice (6-8 weeks, male and female), 32 days post viral infection. Spleen and blood were treated with ACK lysis buffer. Kidneys, salivary gland, liver, and fat were digested with collagenase. Lymphocytes from the small intestine, kidney, salivary gland, and liver were separated on a 44%/67% Percoll density gradient. P14 cells were sorted from spleen and blood. IV- P14 cells were sorted from kidney, salivary gland, IELs, liver, and fat. The sorted cells were taken for encapsulation and RNAseq using the 10x Chromium platform.

Data taken from Crowl et al., Nature Immunology (2022).