We downloaded a single-cell RNA-sequencing (scRNA-seq) dataset from the Gene Expression Omnibus (GEO) under accession number GSE139125 and followed the Seurat pipeline to integrate and identify clusters within two conditions. Based on the canonical marker genes used by Alshetaiwi et al., we successfully identified T cells, B cells, monocytes, monocytic myeloid-derived suppressor cells (M-MDSCs), neutrophils, and granulocytic myeloid-derived suppressor cells (G-MDSCs).
