Briefly, fourteen four-week-old commercial mixed breed pigs seronegative for antibodies against H1N1, H3N2, and B influenza viruses were intratracheally inoculated with 1 x 106 TCID50 2009 pandemic H1N1 A/California/04/2009 influenza virus. Seven of these pigs were orally administered 75 mg oseltamivir phosphate (Lupin Pharmaceuticals) twice a day for five days after infection (FLU/OTV group) while the remaining seven pigs were left untreated (FLU group). This object was used to unidentify the transcriptional changes induced by influenza infection with and without oseltamivir treatment.