Single-cell RNA sequencing was performed on cells isolated from milk and blood of three Holstein cows with chronic mastitis caused by experimental Staphylococcus aureus infection. Cells were classified into 62 clusters and annotated as 6 different cell types. A data subset of only granulocytes was also created and further investigated. 

Data found here are associated with single-cell RNA sequencing (scRNA-seq) analysis of the below work:

Single-cell RNA sequencing characterization of Holstein cattle blood and milk immune cells during a chronic Staphylococcus aureus mastitis infection by Jayne E. Wiarda, Kaitlyn M. Sarlo Davila, Julian M. Trachsel, Crystal L. Loving, Paola Boggiotto, John D. Lippolis, and Ellie J. Putz

Preprint link: TBD

Peer-reviewed publication: TBD this work has not been accepted for peer-reviewed publication and is still subject to alterations

Additional data associated with this project can also be found as detailed below:

• Raw sequencing data can be found in the Sequence Read Archive (SRA) under BioProject ID PRJNA1114020 (the sequencing data will be released upon publication)
• Processed data objects can be found at ______ (data will be available for download upon publication)
• Scripts used for data analysis can be found at https://github.com/SwiVi/scRNAseq_ChronicMastitis_MilkBlood