We collected abdominal subcutaneous adipose tissue (ASAT) samples from a cohort of individuals with obesity enrolled for bariatric surgery at three key time points:
- 1) at the time of enrollment (visit 1).
- 2) at the time of surgery following a mandatory lifestyle-induced weight loss (8-10%) (visit 2).
- 3) following a dramatic weight loss (20-45%) two years post-surgery (visit 3).
For single nuclei RNA-sequencing, nuclei were isolated and pooled from 14 donors (7 females and 7 males) at each visit. After genetic demultiplexing and rigorous quality control, we identified a total of 130.672 nuclei, representing 9 major cell types and 26 subpopulations in ASAT.
