Spatial and molecular characteristics determine tissue function, yet high-resolution methods to capture both concurrently are lacking. Here, we developed high-definition spatial transcriptomics, which captures RNA from histological tissue sections on a dense, spatially barcoded bead array. Each experiment recovers several hundred thousand transcript-coupled spatial barcodes at 2-μm resolution, as demonstrated in mouse brain and primary breast cancer. This opens the way to high-resolution spatial analysis of cells and tissues.

For details and file descriptions, please go to our github repo at: For questions and feedback, please email Sanja Vickovic (



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Related publications
High-definition spatial transcriptomics for in situ tissue profiling
Nature Methods volume 16, pages987–990 (2019)